Publications

Asencio C, Hervé P, Morand P, Oliveres Q, Morel CA, Prouzet-Mauleon V, Biran M, Monic S, Bonhivers M, Robinson DR, Ouellette M, Rivière L, Bringaud F, Tetaud E. Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. Mol Microbiol. 2024 121, 1079-1094. PubMed PMID:38558208

Taleva G, Husová M, Panicucci B, Hierro-Yap C, Pineda E, Biran M, Moos M, Šimek P, Butter F, Bringaud F, Zíková A. Mitochondrion of the Trypanosoma brucei long slender bloodstream form is capable of ATP production by substrate-level phosphorylation. PLoS Pathog. 2023 19, e1011699. PubMed PMID:37819951

Hervé P, Monic S, Bringaud F, Rivière L. Phospholipases A and Lysophospholipases in protozoan parasites. Microb Cell. 2023 10, 204-216. PubMed PMID:37786811

Trindade S, De Niz M, Costa-Sequeira M, Bizarra-Rebelo T, Bento F, Dejung M, Narciso MV, López-Escobar L, Ferreira J, Butter F, Bringaud F, Gjini E, Figueiredo LM. Slow growing behavior in African trypanosomes during adipose tissue colonization. Nat Commun. 2022 13, 7548. PubMed PMID:36481558

Monic SG, Lamy A, Thonnus M, Bizarra-Rebelo T, Bringaud F, Smith TK, Figueiredo LM, Rivière L. A novel lipase with dual localisation in Trypanosoma brucei. Sci Rep. 2022 12, 4766. PubMed PMID:35306507

Tounkara M, Boulangé A, Thonnus M, Bringaud F, Bélem AMG, Bengaly Z, Thévenon S, Berthier D, Rivière L. Novel protein candidates for serodiagnosis of African animal trypanosomosis: Evaluation of the diagnostic potential of lysophospholipase and glycerol kinase from Trypanosoma brucei. PLoS Negl Trop Dis. 2021 15, e0009985. PubMed PMID:34919562

Bringaud F, Plazolles N, Pineda E, Asencio C, Villafraz O, Millerioux Y, Rivière L, Tetaud E. Glycerol, a possible new player in the biology of trypanosomes. PLoS Pathog. 2021 17, e1010035. PubMed PMID:34855923

Oldenburg SH, Buisson L, Beneyton T, Pekin D, Thonnus M, Bringaud F, Rivière L, Baret JC. Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation. Sci Rep. 2021 11, 18192. PubMed PMID:34521865

Allmann S, Wargnies M, Plazolles N, Cahoreau E, Biran M, Morand P, Pineda E, Kulyk H, Asencio C, Villafraz O, Rivière L, Tetaud E, Rotureau B, Mourier A, Portais JC, Bringaud F. Glycerol suppresses glucose consumption in trypanosomes through metabolic contest. PLoS Biol. 2021 19, e3001359. PubMed PMID:34388147

Villafraz O, Baudouin H, Mazet M, Kulyk H, Dupuy JW, Pineda E, Botté C, Inaoka DK, Portais JC, Bringaud F. The Trypanosome UDP-Glucose Pyrophosphorylase Is Imported by Piggybacking into Glycosomes, Where Unconventional Sugar Nucleotide Synthesis Takes Place. mBio. 2021 12, e0037521. PubMed PMID:34044588

Souza ROO, Damasceno FS, Marsiccobetre S, Biran M, Murata G, Curi R, Bringaud F, Silber AM. Fatty acid oxidation participates in resistance to nutrient-depleted environments in the insect stages of Trypanosoma cruzi. PLoS Pathog. 2021 17, e1009495. PubMed PMID:33819309

Michels PAM, Villafraz O, Pineda E, Alencar MB, Cáceres AJ, Silber AM, Bringaud F. Carbohydrate metabolism in trypanosomatids: New insights revealing novel complexity, diversity and species-unique features. Exp Parasitol. 2021 224, 108102. PubMed PMID:33775649

Schenk R, Bachmaier S, Bringaud F, Boshart M. Efficient flavinylation of glycosomal fumarate reductase by its own ApbE domain in Trypanosoma brucei. FEBS J. 2021 288, 5430-5445. PubMed PMID:33755328

Wargnies M, Plazolles N, Schenk R, Villafraz O, Dupuy JW, Biran M, Bachmaier S, Baudouin H, Clayton C, Boshart M, Bringaud F. Metabolic selection of a homologous recombination-mediated gene loss protects Trypanosoma brucei from ROS production by glycosomal fumarate reductase. J Biol Chem. 2021 296, 100548. PubMed PMID:33741344

Villafraz O, Biran M, Pineda E, Plazolles N, Cahoreau E, Ornitz Oliveira Souza R, Thonnus M, Allmann S, Tetaud E, Rivière L, Silber AM, Barrett MP, Zíková A, Boshart M, Portais JC, Bringaud F. Procyclic trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in the presence of physiological amounts of proline. PLoS Pathog. 2021 17, e1009204. PubMed PMID:33647053

Mochizuki K, Inaoka DK, Mazet M, Shiba T, Fukuda K, Kurasawa H, Millerioux Y, Boshart M, Balogun EO, Harada S, Hirayama K, Bringaud F, Kita K. The ASCT/SCS cycle fuels mitochondrial ATP and acetate production in Trypanosoma brucei. Biochim Biophys Acta Bioenerg. 2020 1861, 148283. PubMed PMID:32763239

Su X, Rak M, Tetaud E, Godard F, Sardin E, Bouhier M, Gombeau K, Caetano-Anollés D, Salin B, Chen H, di Rago JP, Tribouillard-Tanvier D. Deregulating mitochondrial metabolite and ion transport has beneficial effects in yeast and human cellular models for NARP syndrome. Hum Mol Genet. 2019 28, 3792-3804. PubMed PMID:31276579

Courtois P, Nabos P, Nzoumbou-Boko R, Reix CE, Dauchy FA, Daulouede S, Bringaud F, Robinson DR, Vincendeau P. Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns). J Vis Exp. 2019 , . PubMed PMID:31009012

Florini F, Naguleswaran A, Gharib WH, Bringaud F, Roditi I. Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei. Nucleic Acids Res. 2019 47, 1725-1739. PubMed PMID:30544263

Fernandes PM, Kinkead J, McNae IW, Bringaud F, Michels PAM, Walkinshaw MD. The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction. Biochem J. 2019 476, 179-191. PubMed PMID:30404924

Wargnies M, Bertiaux E, Cahoreau E, Ziebart N, Crouzols A, Morand P, Biran M, Allmann S, Hubert J, Villafraz O, Millerioux Y, Plazolles N, Asencio C, Rivière L, Rotureau B, Boshart M, Portais JC, Bringaud F. Gluconeogenesis is essential for trypanosome development in the tsetse fly vector. PLoS Pathog. 2018 14, e1007502. PubMed PMID:30557412

Pineda E, Thonnus M, Mazet M, Mourier A, Cahoreau E, Kulyk H, Dupuy JW, Biran M, Masante C, Allmann S, Rivière L, Rotureau B, Portais JC, Bringaud F. Glycerol supports growth of the Trypanosoma brucei bloodstream forms in the absence of glucose: Analysis of metabolic adaptations on glycerol-rich conditions. PLoS Pathog. 2018 14, e1007412. PubMed PMID:30383867

Millerioux Y, Mazet M, Bouyssou G, Allmann S, Kiema TR, Bertiaux E, Fouillen L, Thapa C, Biran M, Plazolles N, Dittrich-Domergue F, Crouzols A, Wierenga RK, Rotureau B, Moreau P, Bringaud F. De novo biosynthesis of sterols and fatty acids in the Trypanosoma brucei procyclic form: Carbon source preferences and metabolic flux redistributions. PLoS Pathog. 2018 14, e1007116. PubMed PMID:29813135

Marchese L, Nascimento JF, Damasceno FS, Bringaud F, Michels PAM, Silber AM. The Uptake and Metabolism of Amino Acids, and Their Unique Role in the Biology of Pathogenic Trypanosomatids. Pathogens. 2018 7, . PubMed PMID:29614775

Kovářová J, Pountain AW, Wildridge D, Weidt S, Bringaud F, Burchmore RJS, Achcar F, Barrett MP. Deletion of transketolase triggers a stringent metabolic response in promastigotes and loss of virulence in amastigotes of Leishmania mexicana. PLoS Pathog. 2018 14, e1006953. PubMed PMID:29554142

Garcia-Silva MR, Sollelis L, MacPherson CR, Stanojcic S, Kuk N, Crobu L, Bringaud F, Bastien P, Pagès M, Scherf A, Sterkers Y. Identification of the centromeres of Leishmania major: revealing the hidden pieces. EMBO Rep. 2017 18, 1968-1977. PubMed PMID:28935715

Smith TK, Bringaud F, Nolan DP, Figueiredo LM. Metabolic reprogramming during the Trypanosoma brucei life cycle. F1000Res. 2017 6, . PubMed PMID:28620452

Thonnus M, Guérin A, Rivière L. A multigene family encoding surface glycoproteins in Trypanosoma congolense. Microb Cell. 2017 4, 90-97. PubMed PMID:28357394

de Taffin de Tilques M, Tribouillard-Tanvier D, Tétaud E, Testet E, di Rago JP, Lasserre JP. Overexpression of mitochondrial oxodicarboxylate carrier (ODC1) preserves oxidative phosphorylation in a yeast model of Barth syndrome. Dis Model Mech. 2017 10, 439-450. PubMed PMID:28188263

Allmann S, Bringaud F. Glycosomes: A comprehensive view of their metabolic roles in T. brucei. Int J Biochem Cell Biol. 2017 85, 85-90. PubMed PMID:28179189

Mantilla BS, Marchese L, Casas-Sánchez A, Dyer NA, Ejeh N, Biran M, Bringaud F, Lehane MJ, Acosta-Serrano A, Silber AM. Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector. PLoS Pathog. 2017 13, e1006158. PubMed PMID:28114403

Harijan RK, Kiema TR, Syed SM, Qadir I, Mazet M, Bringaud F, Michels PAM, Wierenga RK. Crystallographic substrate binding studies of Leishmania mexicana SCP2-thiolase (type-2): unique features of oxyanion hole-1. Protein Eng Des Sel. 2017 30, 225-233. PubMed PMID:28062645

Surve SV, Jensen BC, Heestand M, Mazet M, Smith TK, Bringaud F, Parsons M, Schnaufer A. NADH dehydrogenase of Trypanosoma brucei is important for efficient acetate production in bloodstream forms. Mol Biochem Parasitol. 2017 211, 57-61. PubMed PMID:27717801

Morales J, Hashimoto M, Williams TA, Hirawake-Mogi H, Makiuchi T, Tsubouchi A, Kaga N, Taka H, Fujimura T, Koike M, Mita T, Bringaud F, Concepción JL, Hashimoto T, Embley TM, Nara T. Differential remodelling of peroxisome function underpins the environmental and metabolic adaptability of diplonemids and kinetoplastids. Proc Biol Sci. 2016 283, . PubMed PMID:27170716

Harijan RK, Mazet M, Kiema TR, Bouyssou G, Alexson SE, Bergmann U, Moreau P, Michels PA, Bringaud F, Wierenga RK. The SCP2-thiolase-like protein (SLP) of Trypanosoma brucei is an enzyme involved in lipid metabolism. Proteins. 2016 84, 1075-96. PubMed PMID:27093562

Štáfková J, Mach J, Biran M, Verner Z, Bringaud F, Tachezy J. Mitochondrial pyruvate carrier in Trypanosoma brucei. Mol Microbiol. 2016 100, 442-56. PubMed PMID:26748989

Jackson AP, Otto TD, Aslett M, Armstrong SD, Bringaud F, Schlacht A, Hartley C, Sanders M, Wastling JM, Dacks JB, Acosta-Serrano A, Field MC, Ginger ML, Berriman M. Kinetoplastid Phylogenomics Reveals the Evolutionary Innovations Associated with the Origins of Parasitism. Curr Biol. 2016 26, 161-172. PubMed PMID:26725202

Creek DJ, Mazet M, Achcar F, Anderson J, Kim DH, Kamour R, Morand P, Millerioux Y, Biran M, Kerkhoven EJ, Chokkathukalam A, Weidt SK, Burgess KE, Breitling R, Watson DG, Bringaud F, Barrett MP. Probing the metabolic network in bloodstream-form Trypanosoma brucei using untargeted metabolomics with stable isotope labelled glucose. PLoS Pathog. 2015 11, e1004689. PubMed PMID:25775470

Bringaud F, Biran M, Millerioux Y, Wargnies M, Allmann S, Mazet M. Combining reverse genetics and nuclear magnetic resonance-based metabolomics unravels trypanosome-specific metabolic pathways. Mol Microbiol. 2015 96, 917-26. PubMed PMID:25753950

Bringaud F, Rogers M, Ghedin E. Identification and analysis of ingi-related retroposons in the trypanosomatid genomes. Methods Mol Biol. 2015 1201, 109-22. PubMed PMID:25388110

Shameer S, Logan-Klumpler FJ, Vinson F, Cottret L, Merlet B, Achcar F, Boshart M, Berriman M, Breitling R, Bringaud F, Bütikofer P, Cattanach AM, Bannerman-Chukualim B, Creek DJ, Crouch K, de Koning HP, Denise H, Ebikeme C, Fairlamb AH, Ferguson MA, Ginger ML, Hertz-Fowler C, Kerkhoven EJ, Mäser P, Michels PA, Nayak A, Nes DW, Nolan DP, Olsen C, Silva-Franco F, Smith TK, Taylor MC, Tielens AG, Urbaniak MD, van Hellemond JJ, Vincent IM, Wilkinson SR, Wyllie S, Opperdoes FR, Barrett MP, Jourdan F. TrypanoCyc: a community-led biochemical pathways database for Trypanosoma brucei. Nucleic Acids Res. 2015 43, D637-44. PubMed PMID:25300491

Allmann S, Mazet M, Ziebart N, Bouyssou G, Fouillen L, Dupuy JW, Bonneu M, Moreau P, Bringaud F, Boshart M. Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei. PLoS One. 2014 9, e114628. PubMed PMID:25493940

Deramchia K, Morand P, Biran M, Millerioux Y, Mazet M, Wargnies M, Franconi JM, Bringaud F. Contribution of pyruvate phosphate dikinase in the maintenance of the glycosomal ATP/ADP balance in the Trypanosoma brucei procyclic form. J Biol Chem. 2014 289, 17365-78. PubMed PMID:24794874

Porcel BM, Denoeud F, Opperdoes F, Noel B, Madoui MA, Hammarton TC, Field MC, Da Silva C, Couloux A, Poulain J, Katinka M, Jabbari K, Aury JM, Campbell DA, Cintron R, Dickens NJ, Docampo R, Sturm NR, Koumandou VL, Fabre S, Flegontov P, Lukeš J, Michaeli S, Mottram JC, Szöőr B, Zilberstein D, Bringaud F, Wincker P, Dollet M. The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. PLoS Genet. 2014 10, e1004007. PubMed PMID:24516393

Mazet M, Morand P, Biran M, Bouyssou G, Courtois P, Daulouède S, Millerioux Y, Franconi JM, Vincendeau P, Moreau P, Bringaud F. Revisiting the central metabolism of the bloodstream forms of Trypanosoma brucei: production of acetate in the mitochondrion is essential for parasite viability. PLoS Negl Trop Dis. 2013 7, e2587. PubMed PMID:24367711

Škodová I, Verner Z, Bringaud F, Fabian P, Lukeš J, Horváth A. Characterization of two mitochondrial flavin adenine dinucleotide-dependent glycerol-3-phosphate dehydrogenases in Trypanosoma brucei. Eukaryot Cell. 2013 12, 1664-73. PubMed PMID:24142106

Millerioux Y, Ebikeme C, Biran M, Morand P, Bouyssou G, Vincent IM, Mazet M, Riviere L, Franconi JM, Burchmore RJ, Moreau P, Barrett MP, Bringaud F. The threonine degradation pathway of the Trypanosoma brucei procyclic form: the main carbon source for lipid biosynthesis is under metabolic control. Mol Microbiol. 2013 90, 114-29. PubMed PMID:23899193

Capewell P, Monk S, Ivens A, Macgregor P, Fenn K, Walrad P, Bringaud F, Smith TK, Matthews KR. Regulation of Trypanosoma brucei Total and Polysomal mRNA during Development within Its Mammalian Host. PLoS One. 2013 8, e67069. PubMed PMID:23840587

Allmann S, Morand P, Ebikeme C, Gales L, Biran M, Hubert J, Brennand A, Mazet M, Franconi JM, Michels PA, Portais JC, Boshart M, Bringaud F. Cytosolic NADPH homeostasis in glucose-starved procyclic Trypanosoma brucei relies on malic enzyme and the pentose phosphate pathway fed by gluconeogenic flux. J Biol Chem. 2013 288, 18494-505. PubMed PMID:23665470